caspase 3 inhibitor ac devd cho  (MedChemExpress)

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-Mts1 complex on L929 cells after 3 ( a ) and 24 ( c ) hours in the presence of caspase 3 and 8 inhibitors and necrostatin1. n = 5 for each point, (* p -value < 0.05). ( b ) Western blot with antibodies to caspase 3 from cell lysates 1 h after 17.1-Mts1 addition (left) and control cells (right). ( d ) Western blot with antibodies to phospho-MLKL, RIP3, and phospho-RIP3 from cell lysates 1 h after addition of 17.1-Mts1 ( left ) and control cells ( right ).

Article Snippet: The agents used to block the cytotoxic activity were added 1 h prior to cell incubation with the 17.1-M7 (1 nM) complex were as follows: caspase 3 inhibitor Ac-DEVD-CHO (5 μM), calpain (10 μM), caspase 8 inhibitor Ac-IEID-CHO (5 μM), RIP1 kinase inhibitor necrostatin 1 (5 μM), EGTA (2 μM), ionol (1 μM), cathepsin B inhibitor Ca-074Me (10 μM), and cathepsin D inhibitor Pepstatin A (10 μM) (all from Thermo Fisher Scientific, Boston, MA, USA).

Techniques: Activity Assay, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-M7 complex on L929 cells after 3 ( a ) and 20 ( b ) hours in the presence of caspase 3 and 8 inhibitors, necrostatin1, EGTA, calpains and cathepsins inhibitors, and antioxidant ionol. n = 5 for each point, (* p -value < 0.05).

Article Snippet: The agents used to block the cytotoxic activity were added 1 h prior to cell incubation with the 17.1-M7 (1 nM) complex were as follows: caspase 3 inhibitor Ac-DEVD-CHO (5 μM), calpain (10 μM), caspase 8 inhibitor Ac-IEID-CHO (5 μM), RIP1 kinase inhibitor necrostatin 1 (5 μM), EGTA (2 μM), ionol (1 μM), cathepsin B inhibitor Ca-074Me (10 μM), and cathepsin D inhibitor Pepstatin A (10 μM) (all from Thermo Fisher Scientific, Boston, MA, USA).

Techniques: Activity Assay